Arsenic Modulates Posttranslational S-Nitrosylation and Translational Proteome in Keratinocytes

Table 1

Identification of arsenic-modulated proteins with nLC-MS/MS.

Spot number	Protein name^a	Accession number^b	MW (kDa)/pI Thero.^c	MW (kDa)/pI Exp.^d	Sequence coverage (%)	MOWSE score	Peptides Matched
287	Transformation upregulated nuclear protein	460789	51.0/5.1	54.5/4.9	12	268	3
631	26S proteasome non-ATPase regulatory subunit 13 isoform 1	157502193	42.9/5.5	44.3/5.2	19	380	8
720	60S acidic ribosomal protein P0	4506667	34.2/5.7	39.2/5.0	18	326	7
735	60S acidic ribosomal protein P0	4506667	34.2/5.7	39.2/6.2	29	422	7
749	60S acidic ribosomal protein P0	4506667	34.2/5.7	38.9/6.5	36	636	9
858	Cathepsin D preproprotein	4503143	44.5/6.1	30.4/5.3	25	422	9
899	NADH-Ubiquinone reductase	4758788	30.2/6.9	26.4/5.8	40	575	8
905	Enoyl-CoA hydratase	1922287	31.3/8.3	26.4/6.1	16	180	3

aFunction of the protein obtained via the MASCOT software (http://www.matrixscience.com) search program by querying the NCBI database. The parameters were set at peptide mass tolerance ±0.4 Da and allowed missed cleavage 1.
^bAccession number from NCSI database.
^cTheoretic protein molecular weight and pI annotated in NCBI database.
^dExperimental protein molecular weight and pI calculated from 2-DE gel.