Research Article

Kinetics of Mushroom Tyrosinase and Melanogenesis Inhibition by N-Acetyl-pentapeptides

Figure 4

Effects of Ac-P4 on cell viability and melanin content of B16F10. (a) Effects of Ac-P4 on cell viability of B16F10 cells. B16F10 cells were treated with various concentrations of Ac-P4 for 24 h. Cell viability was determined using an MTT assay. Each percentage value in treated cells was calculated and compared with that in control cells. (b) Effects of Ac-P4 and kojic acid on cellular melanin content in B16F10 melanoma cells. The control readings were set at 100%. Data from experimental wells are expressed as percentages of the controls. Each column represents the mean ± SD of 3 independent experiments. , compared with the α-MSH alone. (c) Reillustrated inhibitory effects of Ac-P4 and kojic acid on cellular melanin content of B16F10 in molarity (mM).
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