Research Article
Response Surface Methodology: Optimisation of Antifungal Bioemulsifier from Novel Bacillus thuringiensis
Table 2
Experimental design—RSM for media optimisation.
| Run order | Point type | Block | BHB | BE | Glycerol | SF oil | 1 average |
| 1 | 1 | 1 | 1 | 3 | 4 | 4 | 38 | 2 | 0 | 1 | 2 | 2 | 3 | 3 | 42.31 | 3 | 1 | 1 | 1 | 1 | 2 | 4 | 50 | 4 | −1 | 1 | 2 | 2 | 2 | 3 | 37 | 5 | 1 | 1 | 1 | 1 | 4 | 4 | 46.15 | 6 | 1 | 1 | 3 | 1 | 2 | 4 | 34.61 | 7 | 1 | 1 | 3 | 1 | 4 | 4 | 40.44 | 8 | 1 | 1 | 3 | 3 | 4 | 2 | 35.71 | 9 | 1 | 1 | 1 | 3 | 2 | 4 | 41.4 | 10 | 0 | 1 | 2 | 2 | 3 | 3 | 40 | 11 | −1 | 1 | 2 | 3 | 3 | 3 | 36 | 12 | 1 | 1 | 1 | 3 | 4 | 2 | 40 | 13 | 0 | 1 | 2 | 2 | 3 | 3 | 42.31 | 14 | 1 | 1 | 1 | 3 | 2 | 2 | 46 | 15 | −1 | 1 | 2 | 2 | 4 | 3 | 36 | 16 | −1 | 1 | 2 | 2 | 3 | 4 | 40 | 17 | −1 | 1 | 3 | 2 | 3 | 3 | 36 | 18 | −1 | 1 | 1 | 2 | 3 | 3 | 42.9 | 19 | 1 | 1 | 3 | 3 | 2 | 4 | 33.33 | 20 | 1 | 1 | 1 | 1 | 2 | 2 | 52 | 21 | 0 | 1 | 2 | 2 | 3 | 3 | 40 | 22 | 0 | 1 | 2 | 2 | 3 | 3 | 40 | 23 | 0 | 1 | 2 | 2 | 3 | 3 | 40 | 24 | 1 | 1 | 3 | 1 | 4 | 2 | 44.44 | 25 | 1 | 1 | 1 | 1 | 4 | 2 | 46 | 26 | −1 | 1 | 2 | 2 | 3 | 2 | 46.15 | 27 | 1 | 1 | 3 | 3 | 4 | 4 | 28 | 28 | 1 | 1 | 3 | 3 | 2 | 2 | 37.33 | 29 | 0 | 1 | 2 | 2 | 3 | 3 | 40 | 30 | −1 | 1 | 2 | 1 | 3 | 3 | 41.94 | 31 | 1 | 1 | 3 | 1 | 2 | 2 | 44.44 |
|
|