Research Article
Expression, Purification, and Functional Analysis of Novel RelE Operon from X. nematophila
Figure 2
Expression profile of recombinant Xn-relE toxin protein. SDS-PAGE showing expression of Xn-relE toxin gene. (a) Lane M, protein marker; lane 1, induced cells harbouring pGEX4T1 vector; lane 2, induced cells from clone 1. (b) Purification of recombinant GST tagged Xn-relE protein by GST affinity chromatography. Lanes 1, 2, and 3, purified recombinant GST tagged Xn-relE protein.
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