Expression, Purification, and Functional Analysis of Novel RelE Operon from X. nematophila

Figure 3

Expression profile of recombinant Xn-relEAT antitoxin protein. SDS-PAGE showing expression of Xn-relEAT protein. (a) Lane M, protein marker; lanes 1, 2, 3, 4, and 5, induced cells from clones 1, 2, 3, 4, and 5; lane 6, induced cells harbouring pET-28 vector. (b) Purification of recombinant His (6x) tagged Xn-relEAT protein by Ni-NTA affinity chromatography. Lane M, protein marker; lane 1, induced cell lysate; lane 2, flow-through; lane 3, wash 1 with 50 mM sodium phosphate buffer; lane 4, wash 2 with 50 mM sodium phosphate buffer containing 20 mM imidazole; lanes 5, 6, 7, and 8, purified recombinant His (6x) tagged Xn-relEAT protein fractions.

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