Anthelmintic Activity of Crude Extract and Essential Oil of Tanacetum vulgare (Asteraceae) against Adult Worms of Schistosoma mansoni
Figure 4
Confocal laser scanning microscopy investigation of the crude hydroalcoholic extract of T. vulgare (TV) in vitro schistosomicidal effect. In these experiments, pairs of adult worms were incubated in 24-well culture plates containing RPMI 1640 medium and treated with different concentrations of TV. After 120 h of incubation or in the case of death, adult male worms were fixed in FAA solution and fluorescent images were obtained using confocal microscopy (Carl Zeiss LSM 510 META). General view of the anterior worm region showing, in red, the location where tegument was analysed (a). Control worms in RPMI 1640 with 0.5% DMSO (b), and 10 μM praziquantel (c), compared to worms treated with 50 μg/mL of TV (d), and 100 μg/mL of TV (e). Scale bars, 500 μm (a) and 50 μm (b–e).
