Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy

Table 1

Settings of PCR cycle for F. hepatica.

Stage	Cycle	Step	Temperature	Time
1	1	1	92°C	3:00 min
2	25	1	92°C	40 sec
		2	50°C	40 sec
		3	72°C	1:00 min
3	1	1	92°C	7:00 min
		2	92°C	Hold