Inhibitory Effect of Cinobufagin on L-Type C<svg  xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" style="vertical-align:-0.2452002pt" id="M1" height="16.138pt" version="1.1" viewBox="-0.0657574 -15.8928 21.8002 16.138" width="21.8002pt"><g transform="matrix(.018,0,0,-0.018,0,0)"><path id="g190-98" d="M433 39L423 65C413 59 399 54 387 54C370 54 352 69 352 114V299C352 352 342 392 307 422C285 440 255 449 225 449C168 437 102 399 75 379C56 365 44 353 44 339C44 315 69 296 87 296C101 296 111 303 116 319C124 349 133 371 145 385C156 397 171 404 190 404C241 404 275 364 275 291V274C253 256 180 229 120 209C65 190 39 159 39 110C39 47 88 -12 159 -12C189 -12 237 25 277 52C282 35 288 21 301 8C312 -3 333 -12 348 -12L433 39ZM275 84C256 65 221 48 195 48C164 48 124 73 124 124C124 161 146 180 185 198C206 208 254 229 275 240V84Z"/><glyph.data ascent="989" descent="-360" horiz-adv-x="439" vert-adv-y="439"/></g><g transform="matrix(.013,0,0,-0.013,7.88,-7.899)"><path id="g190-243" d="M411 139C381 76 368 73 314 73H129L267 224C351 317 400 380 400 469C400 574 322 635 233 635C176 635 128 610 98 576L40 495L63 476C89 515 130 568 198 568C274 568 316 519 316 436C316 347 251 264 191 192C142 134 85 76 30 21V0H403C415 45 425 89 438 131L411 139Z"/><glyph.data ascent="989" descent="-360" horiz-adv-x="465" vert-adv-y="465"/></g><g transform="matrix(.013,0,0,-0.013,13.702,-7.899)"><path id="g190-44" d="M529 230V280H320V487H262V280H52V230H262V0H320V230H529Z"/><glyph.data ascent="989" descent="-360" horiz-adv-x="580" vert-adv-y="580"/></g></svg> Currents, Contractility, and C<svg  xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" style="vertical-align:-0.2452002pt" id="M2" height="16.138pt" version="1.1" viewBox="-0.0657574 -15.8928 21.8002 16.138" width="21.8002pt"><g transform="matrix(.018,0,0,-0.018,0,0)"><use xlink:href="#g190-98"/><glyph.data ascent="989" descent="-360" horiz-adv-x="439" vert-adv-y="439"/></g><g transform="matrix(.013,0,0,-0.013,7.88,-7.899)"><use xlink:href="#g190-243"/><glyph.data ascent="989" descent="-360" horiz-adv-x="465" vert-adv-y="465"/></g><g transform="matrix(.013,0,0,-0.013,13.702,-7.899)"><use xlink:href="#g190-44"/><glyph.data ascent="989" descent="-360" horiz-adv-x="580" vert-adv-y="580"/></g></svg> Homeostasis of Isolated Adult Rat Ventricular Myocytes

<table>Ver (0.1 mM) completely blocks <svg height="15.4375" id="M23" style="vertical-align:-3.25792pt" version="1.1" viewbox="0 0 32.0625 15.4375" width="32.0625" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.112)"><path d="M414 650l-6 -28q-63 -4 -78.5 -17.5t-27.5 -71.5l-77 -416q-11 -58 0.5 -71.5t77.5 -17.5l-5 -28h-275l8 28q63 4 79.5 18.5t27.5 70.5l80 416q11 57 -2 71t-81 18l6 28h273z" id="x1D43C"></path></g>
<g transform="matrix(.012,-0,0,-.012,7.487,15.187)"><path d="M614 175l29 -10q-33 -109 -57 -154q-121 -26 -184 -26q-90 0 -160.5 29t-112.5 77t-63.5 105.5t-21.5 119.5q0 157 108 253t277 96q36 0 71.5 -5t69 -13.5t36.5 -8.5q15 -102 20 -150l-29 -8q-20 79 -66.5 114t-128.5 35q-119 0 -187.5 -86t-68.5 -207
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</svg> in rat ventricular myocytes. (a) Representative <svg height="15.4375" id="M24" style="vertical-align:-3.25792pt" version="1.1" viewbox="0 0 32.0625 15.4375" width="32.0625" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.112)"><path d="M414 650l-6 -28q-63 -4 -78.5 -17.5t-27.5 -71.5l-77 -416q-11 -58 0.5 -71.5t77.5 -17.5l-5 -28h-275l8 28q63 4 79.5 18.5t27.5 70.5l80 416q11 57 -2 71t-81 18l6 28h273z" id="x1D43C"></path></g>
<g transform="matrix(.012,-0,0,-.012,7.487,15.187)"><path d="M614 175l29 -10q-33 -109 -57 -154q-121 -26 -184 -26q-90 0 -160.5 29t-112.5 77t-63.5 105.5t-21.5 119.5q0 157 108 253t277 96q36 0 71.5 -5t69 -13.5t36.5 -8.5q15 -102 20 -150l-29 -8q-20 79 -66.5 114t-128.5 35q-119 0 -187.5 -86t-68.5 -207
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</svg> recordings according to the steady-state activation protocol before and after application of Ver. Summary data of <svg height="15.4375" id="M25" style="vertical-align:-3.25792pt" version="1.1" viewbox="0 0 32.0625 15.4375" width="32.0625" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.112)"><path d="M414 650l-6 -28q-63 -4 -78.5 -17.5t-27.5 -71.5l-77 -416q-11 -58 0.5 -71.5t77.5 -17.5l-5 -28h-275l8 28q63 4 79.5 18.5t27.5 70.5l80 416q11 57 -2 71t-81 18l6 28h273z" id="x1D43C"></path></g>
<g transform="matrix(.012,-0,0,-.012,7.487,15.187)"><path d="M614 175l29 -10q-33 -109 -57 -154q-121 -26 -184 -26q-90 0 -160.5 29t-112.5 77t-63.5 105.5t-21.5 119.5q0 157 108 253t277 96q36 0 71.5 -5t69 -13.5t36.5 -8.5q15 -102 20 -150l-29 -8q-20 79 -66.5 114t-128.5 35q-119 0 -187.5 -86t-68.5 -207
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</svg> before and after application of Ver. (b) <svg height="15.4375" id="M26" style="vertical-align:-3.25792pt" version="1.1" viewbox="0 0 32.0625 15.4375" width="32.0625" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.112)"><path d="M414 650l-6 -28q-63 -4 -78.5 -17.5t-27.5 -71.5l-77 -416q-11 -58 0.5 -71.5t77.5 -17.5l-5 -28h-275l8 28q63 4 79.5 18.5t27.5 70.5l80 416q11 57 -2 71t-81 18l6 28h273z" id="x1D43C"></path></g>
<g transform="matrix(.012,-0,0,-.012,7.487,15.187)"><path d="M614 175l29 -10q-33 -109 -57 -154q-121 -26 -184 -26q-90 0 -160.5 29t-112.5 77t-63.5 105.5t-21.5 119.5q0 157 108 253t277 96q36 0 71.5 -5t69 -13.5t36.5 -8.5q15 -102 20 -150l-29 -8q-20 79 -66.5 114t-128.5 35q-119 0 -187.5 -86t-68.5 -207
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</svg>was recorded under control conditions, during exposure to <svg height="16.049999" id="M27" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 29.762501 16.049999" width="29.762501" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,15.775)"><path d="M384 0h-275v27q67 5 81.5 18.5t14.5 68.5v385q0 38 -7.5 47.5t-40.5 10.5l-48 2v24q85 15 178 52v-521q0 -55 14.5 -68.5t82.5 -18.5v-27z" id="x31"></path></g><g transform="matrix(.017,-0,0,-.017,8.222,15.775)"><path d="M241 635q53 0 94 -28.5t63.5 -76t33.5 -102.5t11 -116q0 -58 -11 -112.5t-34 -103.5t-63.5 -78.5t-94.5 -29.5t-95 28t-64.5 75t-34.5 102.5t-11 118.5q0 58 11.5 112.5t34.5 103t64.5 78t95.5 29.5zM238 602q-32 0 -55.5 -25t-35.5 -68t-17.5 -91t-5.5 -105
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<g transform="matrix(.012,-0,0,-.012,16.375,7.613)"><path d="M535 230h-483v50h483v-50z" id="x2212"></path></g><g transform="matrix(.012,-0,0,-.012,23.36,7.613)"><path d="M249 635q70 0 116 -43t46 -105q0 -46 -28 -80q-22 -25 -80 -64q62 -35 97 -75t35 -99q0 -81 -63 -131t-135 -50q-83 0 -137.5 45.5t-54.5 123.5q0 52 45 95q29 28 89 64q-109 62 -109 155q0 66 50.5 115t128.5 49zM238 603q-42 0 -67.5 -31t-25.5 -72q0 -50 32.5 -79.5
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</svg> M CBG and during wash out. Data are means ± S.E.M. (<svg height="11.225" id="M28" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 36.237499 11.225" width="36.237499" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,10.963)"><path d="M495 86q-46 -47 -87 -72.5t-63 -25.5q-43 0 -16 107l49 210q7 34 8 50.5t-3 21t-13 4.5q-35 0 -109.5 -72.5t-115.5 -140.5q-21 -75 -38 -159q-50 -10 -76 -21l-6 8l84 340q8 35 -4 35q-17 0 -67 -46l-15 26q44 44 85.5 70.5t64.5 26.5q35 0 10 -103l-24 -98h2
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</svg> cells). <svg height="17.825001" id="M29" style="vertical-align:-3.13504pt" version="1.1" viewbox="0 0 76.137497 17.825001" width="76.137497" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.012,-0,0,-.012,2.9,5.525)"><path d="M471 153q-22 -15 -61 -13q-25 31 -45.5 49.5t-56.5 41.5q4 -71 28 -134q-17 -33 -42 -46q-24 12 -42 46q24 63 28 134q-36 -23 -56.5 -41.5t-45.5 -49.5q-36 -2 -61 13q0 28 19 59q65 10 130 43q-65 33 -130 43q-19 31 -19 59q22 15 61 13q25 -31 45.5 -49.5t56.5 -41.5
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<g transform="matrix(.017,-0,0,-.017,17.488,14.875)"><path d="M619 482q0 -69 -40.5 -119t-96 -72.5t-118.5 -26.5h-44l-70 20l-31 -151q-14 -67 0.5 -83t89.5 -22l-5 -28h-287l8 28q65 7 81.5 22t29.5 83l79 398q12 56 0.5 70.5t-78.5 20.5l7 28h255q108 0 164 -43t56 -125zM524 478q0 141 -146 141q-25 0 -47 -8q-16 -6 -20.5 -13.5
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</svg>, versus control.</table>

The Scientific World Journal

fig2

Figure 2

Figure 2: Inhibitory Effect of Cinobufagin on L-Type C<svg  xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" style="vertical-align:-0.2452002pt" id="M1" height="16.138pt" version="1.1" viewBox="-0.0657574 -15.8928 21.8002 16.138" width="21.8002pt"><g transform="matrix(.018,0,0,-0.018,0,0)"><path id="g190-98" d="M433 39L423 65C413 59 399 54 387 54C370 54 352 69 352 114V299C352 352 342 392 307 422C285 440 255 449 225 449C168 437 102 399 75 379C56 365 44 353 44 339C44 315 69 296 87 296C101 296 111 303 116 319C124 349 133 371 145 385C156 397 171 404 190 404C241 404 275 364 275 291V274C253 256 180 229 120 209C65 190 39 159 39 110C39 47 88 -12 159 -12C189 -12 237 25 277 52C282 35 288 21 301 8C312 -3 333 -12 348 -12L433 39ZM275 84C256 65 221 48 195 48C164 48 124 73 124 124C124 161 146 180 185 198C206 208 254 229 275 240V84Z"/><glyph.data ascent="989" descent="-360" horiz-adv-x="439" vert-adv-y="439"/></g><g transform="matrix(.013,0,0,-0.013,7.88,-7.899)"><path id="g190-243" d="M411 139C381 76 368 73 314 73H129L267 224C351 317 400 380 400 469C400 574 322 635 233 635C176 635 128 610 98 576L40 495L63 476C89 515 130 568 198 568C274 568 316 519 316 436C316 347 251 264 191 192C142 134 85 76 30 21V0H403C415 45 425 89 438 131L411 139Z"/><glyph.data ascent="989" descent="-360" horiz-adv-x="465" vert-adv-y="465"/></g><g transform="matrix(.013,0,0,-0.013,13.702,-7.899)"><path id="g190-44" d="M529 230V280H320V487H262V280H52V230H262V0H320V230H529Z"/><glyph.data ascent="989" descent="-360" horiz-adv-x="580" vert-adv-y="580"/></g></svg> Currents, Contractility, and C<svg  xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" style="vertical-align:-0.2452002pt" id="M2" height="16.138pt" version="1.1" viewBox="-0.0657574 -15.8928 21.8002 16.138" width="21.8002pt"><g transform="matrix(.018,0,0,-0.018,0,0)"><use xlink:href="#g190-98"/><glyph.data ascent="989" descent="-360" horiz-adv-x="439" vert-adv-y="439"/></g><g transform="matrix(.013,0,0,-0.013,7.88,-7.899)"><use xlink:href="#g190-243"/><glyph.data ascent="989" descent="-360" horiz-adv-x="465" vert-adv-y="465"/></g><g transform="matrix(.013,0,0,-0.013,13.702,-7.899)"><use xlink:href="#g190-44"/><glyph.data ascent="989" descent="-360" horiz-adv-x="580" vert-adv-y="580"/></g></svg> Homeostasis of Isolated Adult Rat Ventricular Myocytes 

The Scientific World Journal

Inhibitory Effect of Cinobufagin on L-Type C Currents, Contractility, and C Homeostasis of Isolated Adult Rat Ventricular Myocytes

Figure 2