Research Article

Progesterone Increases Apoptosis and Inversely Decreases Autophagy in Human Hepatoma HA22T/VGH Cells Treated with Epirubicin

Figure 4

Epirubicin and/or progesterone effect on autophagy induction. HA22T/VGH cells were treated with epirubicin and/or progesterone for 24 h then stained with acridine orange and followed by flow cytometric analysis of autophagy. Results are expressed as the mean ± standard deviation (S.D.) for three separate experiments. C: untreated cells, E0.03: 0.03 μM epirubicin, E0.1: 0.1 μM epirubicin, E0.3: 0.3 μM epirubicin, and P30: 30 μM progesterone. compared to the untreated controls. # compared to epirubicin-treated group.
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