Optimal Immobilization of <i>β</i>-Galactosidase onto <i>κ</i>-Carrageenan Gel Beads Using Response Surface Methodology and Its Applications

<table class="table-group" id="tab4"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td align="left" rowspan="2"><svg height="16.6625" id="M50" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 10 16.6625" width="10" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>-Galactosidase form</td><td align="center" colspan="2">Kinetic constants</td></tr><tr class="thead"><td align="center"><svg height="15.4" id="M51" style="vertical-align:-3.39066pt" version="1.1" viewbox="0 0 23.325001 15.4" width="23.325001" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>
									 (mM)</td><td align="center"><svg height="15.4" id="M52" style="vertical-align:-3.39066pt" version="1.1" viewbox="0 0 33.950001 15.4" width="33.950001" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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									 (<svg height="12.125" id="M53" style="vertical-align:-3.50804pt" version="1.1" viewbox="0 0 8.8249998 12.125" width="8.8249998" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>mol·min<sup>−1</sup>)</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left">Free</td><td align="center">22.9</td><td align="center">131.2</td></tr><tr><td align="left">Immobilized</td><td align="center">61.6</td><td align="center">177.1</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Kinetic constants of free and immobilized <svg height="16.6625" id="M49" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 10 16.6625" width="10" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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t-62 25.5q-24 0 -43.5 -9t-35 -29.5t-27 -44t-22.5 -63t-19.5 -75.5t-18.5 -91q-57 -294 -68 -380q-26 -190 -35 -200q-26 -31 -97 -37l-4 26q19 9 48 170l77 413q23 121 52.5 187.5t83.5 114.5q70 62 148 62q51 0 88.5 -34t37.5 -91z" id="x1D6FD"></path></g>
</svg>-galactosidase.

The Scientific World Journal

tab4

Table 4

Table 4: Optimal Immobilization of <i>β</i>-Galactosidase onto <i>κ</i>-Carrageenan Gel Beads Using Response Surface Methodology and Its Applications 

The Scientific World Journal

Optimal Immobilization of β-Galactosidase onto κ-Carrageenan Gel Beads Using Response Surface Methodology and Its Applications

Table 4