Optimization (Central Composite Design) and Validation of HPLC Method for Investigation of Emtricitabine Loaded Poly(lactic-co-glycolic acid) Nanoparticles: In Vitro Drug Release and In Vivo Pharmacokinetic Studies

Table 1

Independent variables, dependent variables, and levels of the face centered central composite design.

Factor	Symbol	Level (−1)	Level (0)	Level (+1)
Independent
Methanol concentration (%)	𝐴	10	20	30
Mobile phase pH	𝐵	2.5	3.5	4.0
Dependent
Retention time	𝑌1
Peak resolution	𝑌2
Peak asymmetry	𝑌3