Research Article
Molecular Characterization of Putative Virulence Determinants in Burkholderia pseudomallei
Table 2
Primers used for PCR and construction of mutants and complemented plasmids.
| Gene | Primer name | Nucleotide sequence (5′ to 3′) | Purpose |
| BPSL2033 | 2033IF | AGAACTTCGAGCAATTGCTG | Internal PCR | 2033IR | GAGAGATGACGTTCGGTCTT | Internal PCR | M2033F | GTGAACTGGTACAAAGAAATATCG | Mutant confirmation | M2033R | CACGTTTCTCGGATAGAGC | Mutant confirmation | C2033F | CTAGAGCGCGGCCTCGCG | Complementation of BPSL2033::Km | C2033R | CATCACTCGGCGCAATGAGACTG | Complementation of BPSL2033::Km |
| BP1026B_I2784 | 2784IF | GTCGAGAGTACGGTGTGTTC | Internal PCR | 2784IR | CCTGCGAAATCCTTATCAC | Internal PCR | M2784F | CTGTTTTCTAAGCGTCAGAAG | Mutant confirmation | M2784R | AAATATGCAGGAAATAGCCCG | Mutant confirmation | C2784F | CCTTCGCGCTGATTTGGT | Complementation of BP1026B_I2784::Km | C2784R | CTACTTCGTAGCTTGATGCGCC | Complementation of BP1026B_I2784::Km |
| BP1026B_I2780 | 2780IF | AAACCAGAAGGGCGATTTC | Internal PCR | 2780IR | GCGTTCTTTTAAGAATTGGGTAG | Internal PCR | M2780F | CAGGTACGATTCATGGAACG | Mutant confirmation | M2780R | GGTATTCCGTGACCTGAATGT | Mutant confirmation | C2780F | AGGCTCGGAGTAGTAACACTT | Complementation of BP1026B_I2780::Km | C2780R | CTACTGCCTATGCTGGGGTAT | Complementation of BP1026B_I2780::Km |
| BURPS1106A_A0094 | 94IF | AGTCGGGGGGTACACCTAC | Internal PCR | 94IR | CGACACCGAGGAAAATTTC | Internal PCR | M94F | GTGAATGTCGATCTTGCG | Mutant confirmation | M94R | TCAATCTCCAGCGAGCTT | Mutant confirmation | C94F | TTCTACCAGCGACTTGGC | Complementation of BURPS1106A_A0094::Km | C94R | TCAATCTCCAGCGAGCTT | Complementation of BURPS1106A_A0094::Km |
| BURPS1106A_1131 | 1131IF | AAGGAGTTGGGTACGTCG | Internal PCR | 1131R | CCCTTGTGCCATTGATAG | Internal PCR | pUT-R | TTTGAGTGACACAGGAACAC | Mutant confirmation | C1131F | GTGGTTCAGCCAGGCACG | Complementation of BURPS1106A_1131::Km | C1131R | GTATGTGTCGTCCGCATTTG | Complementation of BURPS1106A_1131::Km |
| BURPS1710A_1419 | 1419IF | GCGATAGCGATTGGAAAACT | Internal PCR | 1419IR | GAATCCAGACCCATTCCGT | Internal PCR | KmF | ATGAGCCATATTCAACGGGA | Mutant confirmation | C1419F | TGCACAAGCTGTTCAAATG | Complementation of BURPS1710A_1419::Km | C1419R | TTAAGGCTTGGGTGCAAG | Complementation of BURPS1710A_1419::Km |
| BPSL3147 | 3147IF | GACCAGTACGCGCTCAAG | Internal PCR | 3147IR | GAACGAATACTTGTCGATCG | Internal PCR | M3147F | GATGTACACGTTCAACGACAAG | Mutant confirmation | M3147R | CTCTTCCGGCATCTCGTA | Mutant confirmation | C3147F | TTCAGGGTTACGAAGCGAAG | Complementation of BPSL3147::Km | C3147R | TCAGTGCAGCCGGATGCTCG | Complementation of BPSL3147::Km |
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