Overexpression of the Synthetic Chimeric Native-T-phylloplanin-GFP Genes Optimized for Monocot and Dicot Plants Renders Enhanced Resistance to Blue Mold Disease in Tobacco (N. tabacum L.)
Figure 5
Assay of blue mold infection in transgenic tobacco plants expressing the nat-T-phylloplanin-GFP gene fusion. Response of mature control tobacco plants (Nicotiana tabacum cv. KY 14) and three transgenic lines (ibm8T1.2, ibm8T.3, and ibm10T2.1) to Peronospora tabacina infection 7 days postinoculation. (a) The untransformed wild-type tobacco cultivar KY14 is highly susceptible to infection. No infection was observed on ibm8T1.2 and ibm8T1.3 plants, and minimal infection was observed on plants of transgenic line ibm10T2.1. (b) Leaves of wild-type KY14 (W) and transgenic (T1.2, T1.3, and T2.1) plants seven days postinoculation with Peronospora tabacina sporangia. (c) Sporulation on leaves of wild-type KY14 (W) and transgenic (T1.2, T1.3, and T2.1) lines seven days postinoculation with P. tabacina. Leaves were removed from the plants and placed overnight inside plastic tubs to initiate sporulation.
