3′ Splice Site Sequences of Spinal Muscular Atrophy Related SMN2 Pre-mRNA Include Enhancers for Nearby Exons
Figure 1
Splicing of SMN1 and SMN2 minigenes recapitulates endogenous splicing in C33A cell line. (a) Minigene constructs of SMN1-S and SMN2-S. The 4719 nt of intron 6 is deleted from both constructs. Primers used in RT-PCR are also shown. (b) RT-PCR analysis of SMN1 and SMN2 minigenes. GAPDH was used as the control. Quantitation results are shown.
