3′ Splice Site Sequences of Spinal Muscular Atrophy Related SMN2 Pre-mRNA Include Enhancers for Nearby Exons
Figure 2
Conserved sequence on 5′ splice site of exon 7 promotes exon 7 inclusion on SMN2 pre-mRNA. (a) A mutant construct (SMN2-E7-5′ss-con) in which 5′ splice site of exon 7 was mutated to the conserved sequence in SMN2 is shown. RT-PCR analysis of SMN2-E7-5′ss-con minigene of SMN2 pre-mRNA. (b) A mutant construct (E7-5′ss-con) in which 5′ splice site of exon 7 was mutated to the conserved sequence in SMN1 is shown. RT-PCR analysis of E7-5′ss-con minigene of SMN1 pre-mRNA is shown.
