3′ Splice Site Sequences of Spinal Muscular Atrophy Related SMN2 Pre-mRNA Include Enhancers for Nearby Exons
Figure 4
3′ splice site of exon 8 also includes an enhancer for exon 7 inclusion. (a) A mutant construct (E8-3′ss-con) in which 3′ splice site of exon 7 was mutated to the conserved sequence in SMN2. RT-PCR analysis of SMN2-E8-3′ss-con minigene of SMN2 pre-mRNA is shown. (b) A mutant construct (SMN1-E8-3′ss-con) in which 5′ splice site of exon 7 was mutated to the conserved sequence in SMN1. RT-PCR analysis of SMN1-E8-3′ss-con minigene of SMN1 pre-mRNA.
