Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells

Figure 2

p53 immunostaining in malignant cells from an invasive (ductal) breast carcinoma. Immunoreactivity specific for p53 was detected in the tumor cell nuclei. Perinuclear p53 immunolocalization is evident. Tumor cell nuclei were more frequently ball-like shaped or displayed an elliptic shape. Sequestered p53 localizes to large aggregates in the cytoplasm (avidin-biotin immunoperoxidase technique with mAb DO-7, counterstained with methyl green, ×320).