The Scientific World Journal

Research Article

Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis

Table 2

Oligonucleotide primers and probes for influenza A virus typing and subtyping in real-time PCR and RT-PCR.
Influenza virus type/subtypePrimer or probesSequence
AAM-151 CATGGAATGGCTAAAGACAAGACC
AM-397AAGTGCACCAGCAGAATAACTGAG
Probe AM-245CTGCAGCGTAGACGCTTTGTCCAAAATG
A/H1N1  
 A/H1HA1-583GGTGTTCATCACCCGTCTAACAT
HA1-895GTGTTTGACACTTCGCGTCACAT
Probe HA1-783TGCCTCAAATATTATTGTGTCCCCGGGT
 A/N1NA1-1078ATGGTAATGGTGTTTGGATAGGAAG
NA1-1352AATGCTGCTCCCACTAGTCCAG
Probe NA1-1138TGATTTGGGATCCTAATGGATGGACAG
A/H3N2  
 A/H3HA3-115GCTACTGAGCTGGTTCAGAGTTC
HA3-375GAAGTCTTCATTGATAAACTCCAG
Probe HA3-208CTATTGGGAGACCCTCATTGTGATGG
 A/N2NA2-560AAGCATGGCTGCATGTTTGTG
NA2-858ACCAGGATATCGAGGATAACAGGA
Probe NA2-821TGCTGAGCACTTCCTGACAATGGGCT
A/H3N8  
 A/H3EqFlu HA3 FTCACATGGACAGGTGTCACTCA
EqFlu HA3 RGGCTGATCCCCTTTTGCA
EqFlu HA3 PrAACGGAAGAAGTGGAGC
 A/N8N8Eq-F30TGG ATC TGC ATC ATT GGG GA
N8eq-R535CTG ACC ATG CCA CCG ATT CA
A/H5N1  
 A/H5H5HA-205-227v2-FCGATCTAGAYGGGGTGAARCCTC
H5HA-326-302v2-RCCTTCTCCACTATGTANGACCATTC
H5-Probe-239-RVaFAM-AGCCAYCCAGCTACRCTACA-MGB
H5-Probe-239-RVbFAM-AGCCATCCCGCAACACTACA-MGB
 A/N1N1-For-474-502TAYAACTCAAGGTTTGAGTCTGTYGCTTG
N1-Rev-603-631ATGTTRTTCCTCCAACTCTTGATRGTGTC
N1-Probe-501-525FAM-TCAGCRAGTGCYTGCCATGATGGCAMGB
AM: M gene of influenza A viruses.
HA1, HA3, and HA5: HA gene of influenza A viruses of subtypes H1, H3, and H5, respectively.
NA, NA2, and NA8: NA gene of influenza A viruses of subtypes N1, N2, and N8, respectively.