Use of Heavy Water (D₂O) in Developing Thermostable Recombinant p26 Protein Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Equine Infectious Anemia Virus Infection

Figure 3

Thermostability of rp26 ELISA at 45°C. Duration of thermostability is indicated by arrow (↓) against strong positive serum (■), medium positive serum (●), and weak positive serum (▲). In 45°C, significant difference exists in 80% D₂O versus 60% D₂O, 𝑃=0.01−0.001, 80% D₂O versus H₂O coating buffer, 𝑃=0.02−0.001, at 2-month storage with respective serum strength.

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