Research Article
Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli
Figure 1
A. glaucus genes confer salt tolerance to E. coli. (a) Spot assay of BL21/pYES2G (empty plasmid), BL21/CCHA-2142, BL21/CCHA-2229, BL21/CCHA-2114, BL21/CCHA-2247, BL21/CCHA-2243, BL21/CCHA-2248, BL21/CCHA-2258, BL21/CCHA-2264, BL21/CCHA-2246, BL21/CCHA-2227, BL21/CCHA-2250, BL21/CCHA-1402, BL21/CCHA-22652, and BL21/CCHA-22654 on LB plates with 0.7 M NaCl and 1 M NaCl. (b) Growth analysis of CCHA-2142, CCHA-2114, CCHA-2229, CCHA-2227, and CCHA-2247 in LB liquid medium with 1 M NaCl. OD600 was recorded every 2 h.
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