Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli

Figure 4

Physiological comparison of control and CCHA-2142-overexpressing Arabidopsis thaliana. (a) Salt tolerance of transgenic plants and WT. Delayed senescence was observed for the transgenic line as compared with WT upon stress with 200 mM NaCl for 3 d. Comparison of SOD activity (b), malondialdehyde (MDA) content (c) and electrical conductivity (d), total chlorophyll content (e), chlorophyll content (f), and chlorophyll content (g) between CCHA-2142-transformed and wild-type Arabidopsis thaliana plants under 200 mM NaCl stress for 1 d, 3 d, and 5 d. 0 d: without stress. Lane CK: negative control (wild-type Arabidopsis thaliana); Lines 2142-1, 2142-6, and 2142-12: transgenic Arabidopsis thaliana lines. Data represent means ± standard deviations (SD) of three biological replicates. One asterisk and two asterisks indicate significant differences at and compared with WT by Student’s -test, respectively.

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