Research Article

Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli

Table 1

Primers used in this study.

PrimerPrimer sequenceDescription

SMART IV oligonucleotide5′-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3′First strand synthesis, forward
CDS III/3′ PCR primer5′-ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)30N–1N-3′ (N = A, G, C, or T; N–1 = A, G, or C)First strand synthesis, reverse
T75′-CGCACAATCCCACTATCCTTCGCAAG-3′Transgenic screening primer, forward
PYES2G-R5′-GATAACATCGCAAGACCGGCAACAGG-3′Transgenic screening primer, reverse
CCHA-42-F5′-CGGACGAAATCCAACCCTTC-3′Real-time PCR primer, forward
CCHA-42-S5′-TTACCCGCGCACTAAGGTCA-3′Real-time PCR primer, reverse
CCHA-14-F5′-CCGGGCAAAAGGAGCATAAA-3′Real-time PCR primer, forward
CCHA-14-S5′-AACCCTGCTCATGCTGGACA-3′Real-time PCR primer, reverse
CCHA-29-F5′-ATTTACGGCCGGGGATATGG-3′Real-time PCR primer, forward
CCHA-29-S5′-ACGGGCCCCATTACCGAATA-3′Real-time PCR primer, reverse
CCHA-21-F5′-GGGTGAATGCGACCHAAACA-3′Real-time PCR primer, forward
CCHA-21-S5′-CCAGCGCTCGATCGTCTTTT-3′Real-time PCR primer, reverse
CCHA-47-F5′-CTTGATGCCCCHACGTTACA-3′Real-time PCR primer, forward
CCHA-47-S5′-TGCGCGCTCAAATGACAAAT-3′Real-time PCR primer, reverse
Actin2-F5′-GCACCCTGTTCTTCTTACCG-3′Standard control primer, forward
Actin2-R5′-AACCCTCGTAGATTGGCACA-3′Standard control primer, forward
Tubulin1-F5′-GAGTTGACCCAGCAGATG-3′Standard control primer, forward
Tubulin1-R5′-CTGGTTCTTGTTCTGGAC-3′Standard control primer, reverse