Research Article

Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli

Table 2

A. glaucus coding sequences expressed during growth on 3 M NaCl. One hundred cDNA clones were randomly selected and sequenced.

A. glaucus unigenesAnnotation (based on similarity to database sequences)

0307, 23171RRM superfamily, nuclear and cytoplasmic polyadenylated RNA-binding protein pub1

2229, 2243, 23119, and 0501ZIP zinc/iron transport family, plasma membrane low affinity zinc ion transporter

0508, 2247, 2372, 2390, 0209, 2302, 2341, 2380, 2382, 23144, 23151, 23159, and 2378Ribosomal_L27e superfamily, 60S ribosomal protein L27e

0509, 23136, and 2018Similar to alpha/beta hydrolase fold-3 domain containing protein

0314Six-hairpin glycosidase

1402, 2037, and 21134HTB_3 (beta-hydroxydecanoyl-acyl carrier protein-(ACP-) dehydratase) structure and subsequently in 4HBT (4-hydroxybenzoyl-CoA thioesterase)

1408, 23140, and 23134RNase_PH superfamily, exosome complex endonuclease 2/ribosomal RNA processing protein

2250MFS general substrate transporter (secondary transporters that include uniporters, symporters, and antiporters)

2330Casein kinase II beta 2 subunit

2334Putative intracellular protease/amidase

2354, 23179, 2042, 2048, 2142, and 2263DUF3431 superfamily

2362WD repeat protein (adaptor/regulatory modules in signal transduction, Pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11–24 residues)

23131p450 superfamily, cytochrome P450 [secondary metabolites biosynthesis, transport, and catabolism/methylsterigmatocystin oxidoreductase]

23149PF03619 domain protein (organic solute transporter Ostalpha)

23207WD40 superfamily. Nuclear pore complex subunit (adaptor/regulatory modules in signal transduction)

2265Spore germination lipase lipC

2251Family 2 glycosyl transferase

2258Phosphoprotein

02037 transmembrane receptors

2301Exodeoxyribonuclease V subunit beta

2302, 2304Protein CRE_17637

2305Aspartic proteinase nepenthesin-1-like

2306Carnitine acetyl transferase

2339TIM_phosphate_binding superfamily, nucleoporin SONB

2377PDI_b_family, PDI_a_family (thioredoxin_like superfamily), disulfide-isomerase precursor

2383, 23163MDR superfamily, delta-aminolevulinic acid dehydratase

23103PING superfamily, regulator of gluconeogenesis Rmd5

23104Fasciclin superfamily, fasciclin domain family protein

23120Lactamase_B superfamily, Zn-dependent hydrolases of the beta-lactamase fold

23135NADH dehydrogenase subunit 1

23187Rho_GDI superfamily (rho-gdp dissociation inhibitor)

23189P-loop NTPase domain superfamily (ras-like GTP-binding protein)

2397SocE

2253, 2227, 2214, 2242, 2369, 23156, 23197, 0215, 0217, and 2346DJ-1/PfpI family protein (Type 1 glutamine amidotransferase- (GATase1-) like domain)

2252Isonitrile hydratase

22651, 2375, 23102, 23143, 23160, 23168, 23191, and 22484-Methyl-5 (B-hydroxyethyl)thiazole monophosphate biosynthesis protein

2243Zinc/iron permease

22652, 2389, and 2246Unknown; no similar proteins found