Differential Diagnosis of Entamoeba spp. in Clinical Stool Samples Using SYBR Green Real-Time Polymerase Chain Reaction

Figure 1

(a) Melting curve graph obtained through the SYBR Green real-time PCR for E. histolytica (A) and E. dispar (B) and the multiplex SYBR Green real-time PCR (C). (b) The software’s graph demonstrating the DNA amplification of E. histolytica and E. dispar mixed DNAs in different amounts at the multiplex SYBR Green real-time PCR in descending order: 183.5 and 198.0 pg (E. histolytica and E. dispar, resp.); 91.5 and 99.0 pg; 45.9 and 49.5 pg; 22.9 and 24.7 pg; 11.5 and 12.4 pg; 8, 5.7 and 6.2 pg; 2.9 and 3.1 pg; 1.4 and 1.6 pg; 0.7 and 0.8 pg; 0.35 and 0.39 pg. (c) Agarose gel from multiplex real-time PCR products. Lane 1, 100 bp ladder standard; lane 2, no sample; lanes 3 to 12, PCR products in descending order of concentration.