Facile and Promising Method for Michael Addition of Indole and Pyrrole to Electron-Deficient trans-β-Nitroolefins Catalyzed by a Hydrogen Bond Donor Catalyst Feist’s Acid and Preliminary Study of Antimicrobial Activity

Table 4

Antimicrobial activity of the newly synthesized compounds against the pathological strains based on well diffusion assay^a.

Comp. no.	Gram-positive bacteria		Gram-negative bacteria		Fungi
	Staphylococcus aureus ATTCC-29213	Bacilils subtilis  ATTCC-10400	Escherichia coli ATTCC-35218	Pseudomonas aeruginosa ATTCC-29336	Candida albicans  ATTCC-10231
7b	N.A.	++	N.A.	N.A.	N.A.
7d	N.A.	++	N.A.	N.A.	N.A.
7e	N.A.	++	N.A.	N.A.	N.A.
7f	N.A.	++	N.A.	N.A.	N.A.
7g	N.A.	++	N.A.	N.A.	N.A.
8e	N.A.	++	N.A.	N.A.	+++
8f	N.A.	++++	N.A.	N.A.	++++
8g	N.A.	++	N.A.	N.A.	++
Ciprofloxacin	+++	++++	++++	++++	N.A.
Ketoconazole	N.A.	N.A.	N.A.	N.A.	++++

aAntimicrobial activities were expressed as inhibition diameter zones in millimeters (mm) as follows: N.A. (no activity) ≤ 4 mm; + (weak) = 5–9 mm; ++ (moderate) = 10–15 mm; +++ (strong) = 16–20 mm; and ++++ (very strong) ≥ 21 mm. The experiment was carried out in triplicate and the average zone of inhibition was calculated.