Direct and Indirect Suppression of Interleukin-6 Gene Expression in Murine Macrophages by Nuclear Orphan Receptor REV-ERBα

Figure 1

REV-ERBα represses il6 gene induction following a LPS challenge in macrophages. (a) Peritoneal macrophages were harvested as adherent cells from 2-month-old C57BL/6J mice and were either untreated or treated with 1 μg/mL LPS or 2 or 20 μM GSK4112 for 16 h. (b) Murine macrophage cell line RAW264 cells were either untreated or treated with 1 μg/mL LPS or 20 μM GSK4112 for 16 h. (c) RAW264 cells transfected with or without rev-erbα were either untreated or treated with 1 μg/mL LPS for 24 h. (d) RAW264 cells transfected with or without rorα were either untreated or treated with 1 μg/mL LPS for 24 h. The gene expression of il6 was analyzed by qPCR. For normalization, actb mRNA was used. The data are presented as the means ± S.E. (). versus cells treated with LPS and without GSK4112 or versus vector control.

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