Direct and Indirect Suppression of Interleukin-6 Gene Expression in Murine Macrophages by Nuclear Orphan Receptor REV-ERBα

Figure 2

REV-ERBα represses NFκB activation in macrophages. (a) Nuclear and cytoplasmic fractions of each cell untreated or treated with LPS for various durations were analyzed by Western blot for p65 NFκB,α-Tub, and TBP. Detection ofα-Tub and TBP is used as marker of nuclear and cytoplasmic fractions, respectively. (b) Each cell was stimulated with LPS for varying durations and NFκB activation was analyzed by EMSA. Data shown are representative of three separate experiments. (c) Each cell was transiently transfected with NFκB-responsive promoter reporter luciferase construct and luciferase activities in each cell stimulated either with or without LPS for 24 h were analyzed. The data are presented as the means ± S.E. from sextuplicate cultures. versus vector control.

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