Effect of Nitrogen Source and Inorganic Phosphate Concentration on Methanol Utilization and PEX Genes Expression in Pichia pastoris

Figure 3

ACP specific activity of P. pastoris strains tr2-1-GS115 (phox PAOX1-PHO5 HIS4) and tr3-1-GS115 (phox PAOX2-PHO5 HIS4) grown in different media: (a) cells were grown in media with different nitrogen sources (MN with ammonium sulphate and MP with proline); (b) cells were grown in media with different concentrations of KH₂PO₄ (MN with 1 g/L and in ML with 0,03 g/L).

(a)

(b)