Research Article

Effect of Nitrogen Source and Inorganic Phosphate Concentration on Methanol Utilization and PEX Genes Expression in Pichia pastoris

Table 1

P.  pastoris strains used in this study.

StrainGenotypeSource

GS115his4 Invitrogen
1-GS115Phox  his4 [10]
tr2-1-GS115Phox  PAOX1-PHO5 HIS4 [10]
tr3-1-GS115phox  PAOX2-PHO5 HIS4 This study
trΔSacI-1-GS115phox  PAOX1ΔSacI-PHO5 HIS4 [10]
trΔNsiI-1-GS115phox  PAOX1ΔNsiI-PHO5 HIS4 [10]
trA-1-GS115phox  PAOX1ΔA-PHO5 HIS4 This study
trB-1-GS115phox PAOX1ΔB-PHO5 HIS4 This study
trC-1-GS115phox PAOX1ΔC-PHO5 HIS4 This study
trD-1-GS115phox PAOX1ΔD-PHO5 HIS4 This study