Effect of Nitrogen Source and Inorganic Phosphate Concentration on Methanol Utilization and PEX Genes Expression in Pichia pastoris

Table 2

Oligonucleotides used in this study.

Primer	Sequence 5^′-3^′
PpACT1F	AGTGTTCCCATCGGTCGTAG
PpACT1R	GGTTCATTGGAGCCTCAGTC
PpDHASF	TACGGATGGGAGAGATACGC
PpDHASR	GTGCTTTGGTTTTCCCTTCA
PpDAKF	TGCCCCAGAAATAGACGAAG
PpDAKR	TTCACCACAATCACCATCTCC
PpPEX5F	CGGAGGAGGCAGTAGAGG
PpPEX5R	AAATGCTCTCTTTAGGGTCTCG
PpFLDF	GAATCTTGCCACAAGGGTTG
PpFLDR	TGCTTTGTTGATGTTGTCCAG
PpFGHF	ATCTCCAACCCCACTAAAGC
PpFGHR	CAACGTGAATCAAAATGCTG
PpPEX8F	TTATGATTTGAATGCCCTCGTC
PpPEX8R	CGGGGTTGTTGTTAAGTAGCTG
PpPEX14F	AATGGTTCGTCCTCAGTTGC
PpPEX14R	TAAAGCCCAAACGAAACACC
PpPEX1F	CGGACAAGGAAGCAAGAAAG
PpPEX1R	TCCCCGACTGAACCATTTAG
ACT1probe	CACCACACCTTCTACAACGAGTTGCGT
DHASprobe	GAGAAGATTGGTGAGAAGGTTGCTG
DAKprobe	TATGCTGACCTTCTGGAGTCTGGT
PEX5probe	GGGCAGATATAAAGAGGCTGTTGAAC
FLDprobe	ATCTCTACCCGTCCTTTCCAGTTGG
PpFGHprobe	GACTCAGTACGACCCAACCGAATTG
PpPEX8probe	TGTGCCTTGGATTACGTTGAAGATG
PpPEX14probe	GCCATCTCCTCCTCCTTTTCCTG
PpPEX1probe	GCTGTTGAAACGGAAGGTTATTTACC
PAOX2F	TTCGACGTCCTGCCCTCTCC
PAOX2R	TTCGGATCCTTTTCTCAGTTGATTTG
PAOXFlaF	ACCTGACGTCTAAGAAACCAT
PAOXFlaR	GCGCGGATCCTTCGAATAATT
delAF	AACGCAAATAGCCTAACG
delAR	TTAGGCTATTTGCGTTTCG
delBF	GGAATACTGTTCTAACC
delBR	GAACAGTATTCCCAC
delCF	GCCTAACGACAACTTGAG
delCR	AAGTTGTCGTTAGGCTATCAG
delDR	GGATCCAGTCGC