Effects of NAA and BAP, Double-Layered Media, and Light Distance on In Vitro Regeneration of Nelumbo nucifera Gaertn. (Lotus), an Aquatic Edible Plant

Table 2

The effect of light distance on the regeneration of shoots. Cultures were maintained at 25±1°C with 16 hours of light and 8 hours of dark, with 1000 lux intensity of light for 4 weeks.

Light distance (mm)	Height of shoot, mm (mean ± SE)	Observations
0.0 (control)	0	Yellow shoots became dried and turned brown
50	9.12±0.51	Yellow shoots turned green
100	10.67±0.09	Yellow shoots turned green
150	13.10±1.01	Yellow shoots turned to green
200	16.67±0.23	Yellow shoots elongated
250	9.13±0.50	Yellow shoots elongated
300	6.33±0.18	Yellow shoots turned brown

Each value represents the mean of thirty replicates. Mean ± standard error (SE), replicates (𝑛=30).