Cellular Localization and Biochemical Characterization of a Chimeric Fluorescent Protein Fusion of Arabidopsis Cellulose Synthase-Like A2 Inserted into Golgi Membrane
Figure 2
AtCslA2-GFP is insoluble in the detergent Triton X-114. Western blot analysis of protein fractions (intracellular, IN; incubation medium, OUT) obtained from AtCslA2-GFP transformed protoplasts (a). AtCslA2-GFP is Triton X-114 insoluble and is mainly recovered in the detergent phase (DET) a small contamination of the chimera was present in the aqueous phase (Aq) (b). Bands were detected using anti-GFP serum.
