Research Article

Blocked Autophagy by miR-101 Enhances Osteosarcoma Cell Chemosensitivity In Vitro

Figure 2

Dox promotes the conversion of LC3-I to LC3-II and increases Atg 5 expression. (a) Western blotting results of U-2 OS cells with Dox (0.1 or 0.2 μg/mL) or Rapa (100 nM) treatment. (b) Quantitative analysis of the conversion of LC3-I to LC3-II in various groups. (c) Quantitative analysis of the relative Agt 5 expression to GAPDH in U-2 OS cells after various treatments. Statistical significance shown as , , and . All results were from three independently performed experiments.
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