Blocked Autophagy by miR-101 Enhances Osteosarcoma Cell Chemosensitivity In Vitro

Figure 3

MiR-101 inhibits the Dox-promoted autophagic vesicles formation in U-2 OS cells. (a) MiR-101 mimics' transfection dramatically elevated the miR-101 level than the transfection of miRNA control. (b) AVOs formed in U-2 OS cells after 50 nM miR control transfection; (c) and (d) AVOs formed in U-2 OS cells after 25 (c) or 50 nM (d) miR-101 mimics transfection. (e) Quantitative analysis of GFP-positive dots (AVOs) in U-2 OS cells. (^∗𝑃<0.05, ^∗∗𝑃<0.01). All experiments were independently performed at least for three times.

(a)

(b)

(c)

(d)

(e)