The Connection between the Toxicity of Anthracyclines and Their Ability to Modulate the P-Glycoprotein-Mediated Transport in A549, HepG2, and MCF-7 Cells
Figure 3
Intracellular DOX and ACL accumulation and their distribution in (a) A549, (b) HepG2, and (c) MCF-7 cell lines. The cells were incubated with IC50 of each anthracycline for 2 h, or, after 2 h incubation with the drugs, the medium was removed and the cells were cultured in fresh medium for another 24 h. In the experiments with Verapamil the cells were preincubated for 30 min at 37°C and then incubated with drugs. Fluorescence was monitored using an Olympus IX70, Japan, magnification 400x.
