A Premature Termination of Human Epidermal Growth Factor Receptor Transcription in Escherichia coli

Figure 2

Western blot analysis of GST-EGFR. Samples from E. coli expressing GST (lane 1) and GST-EGFR (lanes 2–4) were processed by Western blot with the anti-GST antibody. (a) 5 min ECL exposure; (b) 30 min ECL exposure; (c) the protein extract was immobilized on glutathione-Sepharose beads, run on SDS-PAGE, and analyzed by Western blot using the anti-EGFR antibody. (Lane P) Protein extract of E. coli cells transformed with empty pGEX-6P-1. (Lane E) Protein extract of E. coli cells transformed with pGEX-EGFR after immobilization on glutathione beads. Arrows indicate the position of each expressed recombinant protein.

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