A Premature Termination of Human Epidermal Growth Factor Receptor Transcription in Escherichia coli

Figure 3

Identification of the truncated protein by MALDI-TOF/TOF analysis. (a) The gel in the inset shows the purified N-terminal truncated EGFR with its corresponding size. The protein masses of the BioRad markers are indicated in kDa. (b) The spectrum shows different peaks of the MS analysis done by MALDI-TOF. The N-terminal truncated EGFR (50 kDa) was subjected to in-gel trypsin digestion before MALDI-TOF analysis. The arrows show the peaks that served for MS/MS analysis with their corresponding masses used for protein identification. (c) MASCOT results: matched peptides to EGFR sequence are shown in capital bold; the last identified peptide ends at K293.

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