Hydrogen Peroxide Is Involved in Salicylic Acid-Elicited Rosmarinic Acid Production in Salvia miltiorrhiza Cell Cultures

Figure 5

Inhibition of DMTU on exogenous SA in the generation of intracellular H₂O₂ (a) and the biosynthesis of RA (b) in suspension cultured cells. 8-day-old suspension cultured cells treated with DMTU 30 min before exogenous H₂O₂ and/or SA treatment as indicated were harvested at 8 h (for H₂O₂ analysis) and 2 d (for RA analysis) later, and the H₂O₂ and Sal B contents were then determined. The Salicylic acid concentration is 22.5 mg/L. The exogenous H₂O₂ concentration is 10 mM. Values are means of three independent experiments. Bars represented standard errors. Different lowercase letters represent the significant differences between the contents of H₂O₂ (a) and Sal B (b) after each treatment at the level of 5%.

(a)

(b)