Hydrogen Peroxide Is Involved in Salicylic Acid-Elicited Rosmarinic Acid Production in Salvia miltiorrhiza Cell Cultures

Figure 6

Inhibition of IMD on exogenous H₂O₂ and/or SA in the generation of intracellular H₂O₂ and the biosynthesis of RA in suspension cultured cells. 8-day-old suspension cultured cells treated with IMD 30 min before exogenous H₂O₂ and/or SA treatment as indicated were harvested at 8 h (for H₂O₂ analysis) and 2 d (for RA analysis) later, and the H₂O₂ and Sal B contents were then determined. The Salicylic acid concentration is 22.5 mg/L. The exogenous H₂O₂ concentration is 10 mM. The IMD concentration is 100 μM. Values are means of three independent experiments. Bars represented standard errors.