Phytoestrogen α-Zearalanol Improves Memory Impairment and Hippocampal Neurogenesis in Ovariectomized Mice

Figure 2

(a) Photomicrograph of 5-bromo-2-deoxyuridine (BrdU) immunopositive cells (new born neurons) in DG in the hippocampus of control and OVX mice with 17β-E2 or α-ZAL treatment. Fewer BrdU-labeled cells were observed in OVX mice compared to control. 17β-E2 and α-ZAL-treated mice expressed higher BrdU⁺ cells compared to OVX animal. Magnification ×40. (b) Quantification of BrdU immunolabeling cells in DG. 𝑛=4 in each group and the result indicated at least three independent experiments in each animal. ^∗∗𝑃<0.01 versus control group; ^##𝑃<0.01 versus OVX group.

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