HIF-1α had Pivotal Effects on Downregulation of miR-210 Decreasing Viability and Inducing Apoptosis in Hypoxic Chondrocytes

Figure 2

Downregulated miR-210 affected the expression of HIF-1α and HIF-1α-regulated molecules (BCL-xl and PHD-2) in C28/I2 cells on hypoxia and normoxia. Cells was harvested at 24 h and 48 h on hypoxia or on normoxia after being transfected with miR-anti or anticontrol. Then, the qRT-PCR assay was conducted to examine the expression of HIF-1α (b) on normoxia. The same method was utilized to analyze the differences in expression of HIF-1α (c), BCL-xl (d), and PHD-2 (e) mRNA between in cells transfected with miR-anti and that with anticontrol on hypoxia. The expression of miR-210 was also confirmed in these groups (a). The qRT-PCR was performed in three individual experiments. Significant differences were denoted as *𝑃<0.05 and **𝑃<0.01.

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