HIF-1α had Pivotal Effects on Downregulation of miR-210 Decreasing Viability and Inducing Apoptosis in Hypoxic Chondrocytes
Figure 3
Overexpression of miR-210 increased expression of HIF-1α and HIF-1α-regulated molecules (BCL-xl and PHD-2) in hypoxic C28/I2 cells. The expression of HIF-1α (a), Bcl-xL (c), and PHD-2 (d) was examined by qRT-PCR after 48 h transfection of HIF-1α si or miR-210 mimics in hypoxic C28/I2 cells. The knockdown of HIF-1α was verified by western plot in the four groups (b). The experiments were performed in triplicate separately. Comparison was made by ANOVA (* and **).
