HIF-1α had Pivotal Effects on Downregulation of miR-210 Decreasing Viability and Inducing Apoptosis in Hypoxic Chondrocytes

Figure 4

Downregulated miR-210 decreased viability in hypoxic C28/I2 cells depending on HIF-1α. Cell viability was detected after 48 culture by MTT assay after being treated with miR-210 mimics, miR-anti, or HIF-1α si on normoxia or hypoxia. Data of three individual experiments was normalized and statistically analyzed by ANOVA. (a) Cell viability on normoxia. (b) Western plot assay of HIF-1α protein in hypoxic cells treated with miR-210 mimics, miR-anti, or HIF-1α si. (c) Cell viability on hypoxia (*𝑃<0.05, **𝑃<0.01). (d) Expression of miR-210 in hypoxic cells treated with miR-210 mimics, miR-anti, or HIF-1α si (**𝑃<0.01).

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