Studies on Optimization of Growth Parameters for L-Asparaginase Production by Streptomyces ginsengisoli

Table 2

66% of the enzyme activity was found to be extracellular when compared with intracellular enzyme activity.

	Streptomyces ginsengisoli	Enzyme activity; (μmol/mL/min)	Enzyme activity (%)
Extracellular	Cell-free supernatant (before sonication)	2.412±0.19	66
Intracellular	Supernatant (after sonication)	0.888±0.03	25
	Precipitate (after sonication)	0.316±0.12	9

For optimization of enzyme production, extracellular enzyme activity was considered.