The In Vitro Effect of Ivermectin on the Activity of Trehalose Synthesis Pathway Enzymes and Their mRNA Expression in the Muscle of Adult Female Ascaris suum (Nematoda)

Table 1

Primer sequences.

Enzyme	Gene	Primer name	Sequence ()	GenBank accession number; of target sequence
Trehalose-6-phosphate synthase (TPS)	tps1	Forward	CTCGTCGTTGTGCACAGTTT	JF412033.2
		Reverse	GCTGTTCCAGCAGTTCTTCC
	tps2	Forward	CCACATGCGGGTCTTATTCT	JF412034.2
		Reverse	ACTCTCGGTGGCTGCACTAT
Trehalose-6-phosphate phosphatase (TPP)	tpp	Forward	CATGGCACTCTTTGTTGGTG	JI169681.1
		Reverse	AGCGTTATTCAGTGCCTCGT
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	gapdh	Forward	CGGTTGTATCGACGGACTTT	AB058666.1
		Reverse	TGAGGCTTTGACGTTCAGTG