Molecular Characterization of Enterohemorrhagic E. coli O157 Isolated from Animal Fecal and Food Samples in Eastern China

Table 1

Primers for detection of virulence genes in EHEC O157 isolates.

Primers	Sequence (5′→3′)	Annealing temperature/°C	Product size/bp	Reference
rfbEO157-F	AAGATTGCGCTGAAGCCTTTG	55	497	[19]
rfbEO157-R	CATTGGCATCGTGTGGACAG
stx1-F	TGTCGCATAGTGGAACCTCA	53	655	[20]
stx1-R	TGCGCACTGAGAAGAAGAGA
stx2-F	CCATGACAACGGACAGCAGTT	53	780	This study
stx2-R	CCTGTCAACTGAGCACTTTG
eae-F	GGTGAAACTGTTGCCGATCT	50	1382	This study
eae-R	TTGCCATTACGGTCATAGGCG
hlyA-F	ACGATGTGGTTTATTCTGGA	50	167	This study
hlyA-R	CTTCACGTCACCATACATAT