Enhanced Production and Characterization of a Solvent Stable Amylase from Solvent Tolerant Bacillus tequilensis RG-01: Thermostable and Surfactant Resistant

Table 1

Morphological, physiological, and biochemical characteristics of the selected RG-01 isolate.

Characteristics	Results
Morphological tests
Gram’s reaction	+
Shape	Cylindrical
Motility	+
Physiological tests
Growth on NaCl (%) 2.0–8.0	+
Growth at pH 7.0–12.0	+
Growth at temp. 25–95°C	+
Growth under anaerobic condition	+
Biochemical tests
Growth on MacConkey agar	−
Indole test	−
Methyl red test	−
Voges-Proskauer test	+
Citrate utilization	−
H2S production	−
Gelatin hydrolysis	+
Esculin hydrolysis	+
Starch hydrolysis	+
Urea hydrolysis	−
Nitrate reduction	−
Ornithine decarboxylase	−
Lysine decarboxylase	−
Catalase test	+
Oxidase test	+
Arginine decarboxylase	−
Tween-20 hydrolysis	+
Tween-40 hydrolysis	+
Gas production from glucose	−
Acid production from
Dextrose	+
Lactose	−

(+): positive; (−): negative.