Enzymatic Activity Analysis and Catalytic Essential Residues Identification of Brucella abortus Malate Dehydrogenase

Figure 3

Enzymatic analysis of B. abortus His-MDH. One microgram of purified His-MDH was added to the reaction buffer and the enzymatic kinetics was measured. The enzymatic activity was detected as detailed in Materials and Methods. The experiments were performed in triplicate, and the bars represent standard deviations.