Cloning and Characterization of a Flavonol Synthase Gene from Scutellaria baicalensis
Figure 3
The structural model of SbFLS. (a) The overall and detailed views of the structure of SbFLS showing the β jelly-roll fold and presumed catalytic site are modeled using SWISS-MODEL server (http://swissmodel.expasy.org/) [27]. Iron atom is represented by a red dot. Substrates quercetin (QUE) and 2-oxoglutarate (2OG) are indicated in yellow and green. The iron, QUE, and 2OG are positioned by the same structure identified in the AtANS complex (PDB code 1GP6). Possible hydrogen bonds are indicated by dashed lines. (b) Sequence alignment of AtANS and various FLSs from S. baicalensis (SbFLS), V. vinifera (VvFLS), C. unshiu (CuFLS), and A. thaliana (AtFLS). The secondary structure was drawn from the modeled structure of SbFLS. Residues coordinating with an iron atom are represented by blue ovals. Green triangles and red rectangles indicate residues that interact with 2OG and QUE, respectively. The figure was produced using the ESPript server (http://espript.ibcp.fr/ESPript/ESPript/) [38].
