Detection of Pathogenic Escherichia coli and Staphylococcus aureus from Cattle and Pigs Slaughtered in Abattoirs in Vhembe District, South Africa

Table 1

Primer sequences used in multiplex PCR for detection of pathogenic E. coli.

E. coli type	Primer sequences	Product size in bp	References
ETEC (LT)
ETEC 508F	5′-CACACGGAGCTCCTCAGTC-3′	508 bp	[23]
ETEC 508R	5′-CCCCCAGCCTAGCTTAGTTT-3′
ETEC (ST)
ETEC 147F	5′-GCTAAACCAGTAGAGGTCTTCAAAA-3′	147 bp	[23]
ETEC 147R	5′-CCCGGTACAGAGCAGGATTACAACA-3′
EHEC (Stx1)
EHEC 384F	5′-CAGTTAATGTGGTGGCGAAGG-3′	384 bp	[23]
EHEC 384R	5′-CACCAGACAATGTAACCGCTG-3′
EHEC (Stx2)
EHEC 584F	5′-ATCCTATTCCCGGGAGTTACG-3′	584 bp	[23]
EHEC 584R	5′-GCGTCATCGTATACACAGGAGC-3′
EPEC (eae)
EPEC 881F	5′-CCCGAATTCGGCACAAGCATAAGC-3′	881 bp	[23]
EPEC 881R	5′-CCCGGATCCGTCTCGCCAGTATTCG-3′
EPEC (bfpA)
EPEC 300F	5′-GGAAGTCAAATTCATGGGGGTAT-3′	300 bp	[23]
EPEC 300R	5′-GGAATCAGACGCAGACTGGTAGT-3′
EIEC (ipaH)
EIEC 423F	5′-TGGAAAAACTCAGTGCCTCT-3′	423 bp	[23]
EIEC 423R	5′-CCAGTCCGTAAATTCATTCT-3′
EAEC (aatA)
EAEC 650F	5′-CTGGCGAAAGACTGTATCAT-3′	650 bp	[23]
EAEC 650R	5′-CAATGTATAGAAATCCGCTGTT-3′
EAEC (aaic)
EAEC 215F	5′-ATTGTCCTCAGGCATTTCAC-3′	215 bp	[23]
EAEC 215R	5′-ACGACACCCCTGATAAACAA-3′